M U SCLE PHYS IOLOGY Biofluorometer Now more reliable, simplified and affordable The new SI-BF100 is an LED-based fluorometer for life science applications . It is ideally suited for ratiometric calcium detection (FURA-2) and ATPase detection (via NADH fluorescence) . With up to seven LED modules (wavelengths), the SIBF100 covers many fluorometric applications in neuroscience and cell biology . Recent advancements in optics and LED technology simplify ratiometric calcium imaging, making this equipment more affordable . A breakthrough in WPI patented technology allows the SI-BF100 to use wavelengths below 380nm and produce more light in those spectra . This technology significantly cuts the cost of photometric calcium imaging without sacrificing resolution or quality . l LED light sources require less power, give off less heat and are more compact and affordable l Sampling rates up to 1kHz (1,000 ratios/ second maximum) . At lower speeds, signal averaging is used for noise reduction . l Two auto ranging photomultiplier inputs allow you to monitor multiple wavelengths from a single emission output that can be comprised of any wavelength of light for which an LED module is available l Using a separate reference channel, ultra-stable, continuous ratio calculations automatically compensate for LED intensity drift . This ensures less noise and produces more accurate measurements . l Application-specific probes are available for existing tissue baths and cuvette systems . l Ratio noise is <0 .05 peak to peak, drift is less than 0 .1 unit/hour l The warm up time of less than one minute is a dramatic improvement over the common 20–60 minutes required by xenon or mercury light sources l Replace the emission filter easily or change the LED modules to transform the SI-BF100 into a general purpose fluorometer for many other applications ric tiomet & Ra um Calci ase ATP t Perfec for: How it Works Up till now, calcium imaging systems have been required to compensate for errors and noise introduced by the complexity of their design . The systems require mechanical filters and use expensive xenon or mercury light sources . The beauty of the SI-BF100 is its simplicity . The elegance of its design reduces the noise introduced into the system and the errors inherent in traditional designs . output to one or two photomultiplier inputs on the front of the SI-BF100, which are independently filtered for specific wavelengths . This design allows you to monitor multiple wavelengths from a single emission output . The LED light source in the WPI design makes this ratiometric fluorometer more compact, energy efficient and affordable . As added benefits, the low-power light source produces much less heat, and it warms up in less than one minute! Monochromatic LED light sources using WPI patented technology eliminate the need for complex and expensive white This incredible design is not limited to calcium light sources and filter imaging either . By simply replacing wheels . Because the 510nm emission Unique fiber optic coupling probe allows the LED modules filters in front of the for highly efficient transfer of light and can be pulsed, photomultipliers ease of placement. Custom probes fit your sampling with the desired existing systems. frequencies up wavelength filters, to 1,000 cycles per your SI-BF100 becomes a second are possible . general purpose fluorometer for any application you can imagine . Changing a filter involves The LED light source emits specific excitation removing the two screws that hold the filter frequencies which travel through the probe . carriage on the face of the SI-BF100, swapping The excitation light can be comprised of any the filter and reinstalling the integral SMA/filter wavelength of light for which an LED module is carriage . available . The probe returns a single emission BF-100 Biofluorometer OPTIONAL COMPONENTS M3301 Manual Manipulator for securing the probe M10 Magnetic Base SI-BF100 SPECIFICATIONS CALL FOR APPLICATIOn FIBER OPTIC LIGHT INPUT/OUTPUT . . . . . . . . . . . BANDWIDTH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . RATIO NOISE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ANALOG OUTPUT RANGE . . . . . . . . . . . . . . . . . . . ANALOG OUTPUT IMPEDANCE . . . . . . . . . . . . . . . POWER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . WARM UP TIME . . . . . . . . . . . . . . . . . . . . . . . . . . . . DIMENSIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . SMA terminated 1000 ratios/second < 0 .1 peak to peak 0–10V (continuous, equivalent to a ratio 0–10) 100W 12VDC, 0 .5A, (universal power supply, 110/240VAC) <1 minute 3 .5"H x 17"W x 13"D (88 x 431 x 330 mm) 6 World Precision Instruments UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com