HYDroGen peroXiDe & oXYGen SenSor GuiDe ISO-HPO-2 APPLICATION SENSOR DIAMETER RESPONSE TIME DETECTION LIMIT DRIFT SENSITIVITY PHYSIOLOGICAL INTERFERENCE Cell Cultures, etc. 2.0 mm <5 SEC (90%) <100 nM to 100 mM <0.1 pA/min 8 pA/µM none ISO-HPO-100 100 micron <5 SEC (90%) 1 nM to 1 mM <1.0 pA/min 1 pA/nM Contact WPI ISO-HPO-100-L 100 micron <5 SEC (90%) 1 nM to 1 mM <1.0 pA/min 1 pA/nM Contact WPI ISO-OXY-2 Cell Cultures, etc. 2.0 mm <10 SEC (90%) 0.1 % to 100% <1%/min N/A none Tissue/Microvessels Tissue Bath Hydrogen peroxide Sensors Hydrogen Peroxide is produced in biological systems by controlled pathways at low concentrations that impact on cell signaling. At higher concentrations inflammatory cells produce local intense amounts of this oxidant to kill pathogens. In the progress of human disease, uncontrolled formation of hydrogen peroxide from the mitochondrial respiratory chain and enzymes, such as xanthine oxidase, can occur (Prof. Victor Darley-Usmar, Univ. of Alabama, personal communication). Despite the recognized importance of this oxidant in biology real-time measurements at low concentration have been difficult. The hydrogen peroxide sensors developed by WPI are designed to compliment existing high sensitivity fluorescent approaches with direct quantitative measurement in biological samples in the low nM range. Two hydrogen peroxide sensors are currently available. The ISOHPO-2 is a 2.0 mm stainless steel sensor, with replaceable membrane sleeves (#600012) and an internal refillable electrolyte (#100042). The sensor is designed for use in cell cultures and similar applications. iSo-Hpo-2 The ISO-HPO-100 is a 100 micron tip diameter hydrogen peroxide micro sensor designed for use in tissues and similar applications. The sensor design is based on a flexible “activated” carbon fiber sensing electrode coated with a proprietary membrane that enhances hydrogen peroxide detection. iSo-Hpo-100 Both sensors incorporate WPI’s proprietary combination electrode technology whereby the hydrogen peroxide sensing element and separate reference electrode are encased within a single Faradayshielded probe design. This design has been shown to enhance performance during measurements and minimizes overall sensor size. BIOS EN S I NG 600011 iSo-Hpo-2 iSo-Hpo-100 600012 100042 ISO-HPO Startup Kit (recommended with first purchase) 2mm Shielded HPO Sensor & Cable 100 µm HPO Sensor (requires #91580), pkg of 3 Replacement Sleeve Kit for ISO-HPO-2, pkg of 4 ISO-HPO-2 Electrolyte (10 mL) iSo-nop30L iSo-nop70L iSo-Hpo-100-L iSo-nopF200-L10 L-shaped sensors for tissue bath & cell culture studies The ISO-NOP30-L is a unique L-shaped nitric oxide sensor designed specifically for use in tissue bath studies and similar applications (e.g., see WPI’s MYOBATH). The shape of the sensor has been engineered to facilitate placement of the electrode within the lumen of the tissue vessel under study. The ISO-NOP70-L is similar in construction to the ISO-NOP30 but with the advantage of having a flexible tip (70 µm diameter). The ISO-NOPF200-L10 is designed specifically for cell culture studies. oxygen Sensors FORT-100 Transducer Micrometer Connect to Microsensor Cable #15810 The ISO-OXY-2 is a 2.0 mm stainless steel sensor, with replaceable membrane sleeves (#5378) and an internal refillable electrolyte (#7326). The sensor is similar in design to WPI’s popular OXELP oxygen sensor (see page 68). iSo-oXY-2 5377 5378 7326 2 mm Shielded Oxygen Sensor & Cable ISO-OXY Startup Kit (recommended with first purchase) Replacement Electrode Sleeve Kit, pkg of 4 ISO2 Filling Solution (electrolyte) Held in micromanipulator 3 mm carbon fiber 10 mm temperature Sensor The temperature sensor (#ISO-TEMP-2) is based on a 2.0 mm tip diameter high quality miniature platinum RTD (Resistance Temperature Detector) electrode. This design has been shown to provide greater accuracy, stability and interchangeability during temperature measurements than traditional thermistor and thermocouple sensors. The ISO-TEMP-2 is included with the purchase of a system. ISO-TEMP-2 91580 2 mm Platinum RTD Temperature Sensor (requires #91580) Microsensor Adapter Cable L-shaped NO sensor used with Myobath. ISO-NOP30-L NO Sensor, L-Shaped 30-micron (pkg of 3) ISO-NOP-70-L NO Sensor, L-Shaped 70-micron (pkg of 2) ISO-NOPF200-L10 NO Sensor, 200 µm Flexible L-shaped (pkg of 2) ISO-HPO-100-L HPO Sensor, L-Shaped 100-micron (pkg of 2) 91580 Microsensor Adapter Cable World Precision Instruments UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com 56 www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com