“E” “D” “C” A “F” “A” “B” DETAIL A penetrates easier and lasts longer than other manufacturer’s 33 gauge, 10 degree single beveled tips. With a 35 gauge tri-surface beveled tip, the resistance to the penetration becomes even less. Each of our tips undergo a penetration test before leaving the factory to guarantee the best results for our customers. Available Tips 33 gauge: This tip is similar to Hamilton’s 7762 and 7803 series removable needles in both tip length and outer diameter. However, our beveled tip version is shorter, more durable, and penetrates better due to the special trisurface grinding technique. In the past, 33 gauge tips were the smallest size sold by other manufacturers and were frequently cited in literature. However, our new 35 gauge tip is much better for injections involving small animals, especially mice. Compared with Hamilton’ 33 gauge, 10 degree beveled tip, our 35 gauge 25 degree beveled tip can reduce the depth of penetration by almost 80%. The distance between the tip and the upper rim of the opening (section F on Figure 2) is 348 microns for the 33 gauge tip. The distance for our 35 gauge tip is only 230 microns. In addition, the smaller tip size significantly reduces the required penetration force. In nearly all applications, a 33 gauge tip can be replaced with our 35 gauge tip and produce better results. 34 gauge: This is a transitional size between the 33 gauge and 35 gauge. If the 35 gauge is too weak and the 33 gauge is too large, this makes a good alternative. 35 gauge: This was the most popular and preferred tip of most scientists during our field trial. The combination of its strength, length, durability, and clogging resistance creates a balance with very little compromising of the individual properties. It is much smaller than the 33 gauge tip offered by other manufacturers. It is only slightly larger than the 36 gauge tip but is much stronger and less likely to be clogged. Samples can be directly loaded with this tip. Its 5 mm length is sufficient enough for almost all injection applications in mice. 36 gauge: This is the smallest tip that is commercially available. The tip is so small that it can be inserted into the opening of the 33 gauge needle tip. Because this is pushing the limits of what current technology can produce, there are some limitations to consider before using. Its thin diameter makes it necessary Tip Tip Order Tip O.D. Tip I.D. Length Number “A” “B” “C” NF33BV-2 NF34BV-2 NF35BV-2 NF36BV-2 NF33FBV-2 NFQ34-5 NF33BL-2 NF34BL-2 NF35BL-2 NF36BL-2 NF33FBl-2 210 µm 185 µm 135 µm 110 µm 200 µm 160 µm 210 µm 185 µm 135 µm 110 µm 200 µm 115 µm 85 µm 55 µm 35 µm 100 µm 100 µm 115 µm 85 µm 55 µm 35 µm 100 µm 10.0 mm 5.0 mm 5.0 mm 3.0 mm 5.0 mm 55.0 mm 10.0 mm 5.0 mm 5.0 mm 3.0 mm 5.0 mm Total Length “D” 40.0 mm 35.0 mm 35.0 mm 33.0 mm 35.0 mm 75.0 mm 34.0 mm 29.0 mm 29.0 mm 27.0 mm 29.0 mm Shank O.D. “E” 460 µm 460 µm 460 µm 460 µm 460 µm 460 µm 460 µm 460 µm 460 µm 460 µm 460 µm Bevel Total Length Dead “F” Volume ≈348 µm ≈290 µm ≈204 µm ≈156 µm ≈322 µm ≈280 µm ≈348 µm ≈290 µm ≈204 µm ≈156 µm ≈322 µm 1.47 nL 1.39 nL 1.38 nL 1.37 nL 1.40 nL 0.59 nL 1.47 nL 1.39 nL 1.38 nL 1.37 nL 1.40 nL Tip Material Stainless Steel Stainless Steel Stainless Steel Stainless Steel Titanium Alloy Quartz Stainless Steel Stainless Steel Stainless Steel Stainless Steel Titanium Alloy PUMPS, MICROINJECTION Above dimensions apply to blunt tips also. to limit its length to 2.5 to 3.0 mm and still maintain a usable strength. Since the tip ID is in the 25 to 50 micron range, it is very easily clogged. Therefore, only well filtered solutions can be used. Depending on the viscosity of the sample, the user might also need to pre-load the syringe with a regular tip before switching to this tip for injection. We recommend using the 35 gauge tip instead of the 36 gauge unless it is absolutely necessary. Flexifil: The Flexifil tip is made of a titanium alloy. The advantage of this tubing is its durability. This “semi-flexible” tip can be bent up to 90 degrees without damage. It is also much more corrosion resistant than the stainless steel tip. Saline solutions left in the tip will be less likely to clog it. Although this tip is specified as a 33 gauge tip, its outer diameter is slightly smaller than our 33 gauge stainless steel tip. Flexible Quartz Tubing : The flexible quartz tubing tip is made of 160 micron OD polyimide coated quartz tubing with a special adapter sleeve mounted at the end. It is designed for filling glass capillary electrodes or pipettes, just like WPI’s traditional MF34g Microfil. However, unlike the traditional MicroFil, which has about 50 microliters of dead volume in its luer hub, the dead volume of this tip is less than 0.6 microliters. It is useful for loading electrodes with solutions that have a limited volume or are too expensive to waste. NANOFIL NEEDLES NF33BL-2 33 g blunt NanoFil needle (pkg of 2) NF34BL-2 34 g blunt NanoFil needle (pkg of 2) NF35BL-2 35 g blunt NanoFil needle (pkg of 2) NF36BL-2 36 g blunt NanoFil needle (pkg of 2) NF33BV-2 33 g beveled NanoFil needle (pkg of 2) NF34BV-2 34 g beveled NanoFil needle (pkg of 2) NF35BV-2 35 g beveled NanoFil needle (pkg of 2) NF36BV-2 36 g beveled NanoFil needle (pkg of 2) NF33FBL-2 33 g Flexifil blunt NanoFil needle (pkg of 2) NF33FBV-2 33 g Flexifil beveled NanoFil needle (pkg of 2) NF33-36BL Assortment of 4 blunt NanoFil needles NF33-36BV Assortment of 4 beveled NanoFil needles REPLACEMENT PARTS & ACCESSORIES NFINHLD NanoFil Injection Holder SILFLEX-2 SilFlex tubing 35 cm long (pkg of 2) (dead volume = 2.74 µL) NFGSK-5 Spare Silicone gasket for NanoFil & Holder (pkg of 5) NFQ34-5 34 gauge Flexible Quartz Tubing for filling (pkg 5) www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com World Precision Instruments UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com 189