Precision Heater Controller Smart, electrically quiet air heater for live cell imaging systems and custom incubators MICROSCOPES, CAMERAS Maintenance of the desired temperature set-point can now be achieved through the use of a precision, shielded temperature probe coupled to a peripheral Air-Therm heating unit. Precision: Noise is a major concern in any experimental application. WPI’s Air-Therm™ is electrically quiet as well as acoustically quiet). Since the Air-Therm can be placed away from the incubator, any residual electrical and vibrational interference from the heater is virtually eliminated. Accuracy of ±0.1°C can be routinely maintained at the sample site (using a fully programmable differential algorithm to automatically adjust the temperature on the Air-Therm unit). Air-Therm maintains a uniform temperature across the microscope stage (allowing for uniform heating of multiwell dishes). These tight tolerances result in minimal focal drift once sample/incubator equilibrium has been achieved. Glass Bottom Dishes: For high numerical aperture (NA) live-cell imaging, cells must be plated on glass (e.g., WPI’s FluoroDish Glass Bottom Dishes, spotting 0.17 mm glass thickness) or standard cover slips. Since cover slips inherit other problems associated with sterility and accidental damage to expensive microscope condensers, WPI designed new and improved glass bottom dishes (35 mm × 10 mm or 35 mm × 22 mm) that are not cytotoxic. A special adhesive in conjunction with an advanced dish design makes the FluoroDish the only non-cytotoxic glass dish in the world. (See page 164.) This means that cells can actually be cultured for 4 to 5 days in the same dish and then carry the complete cell culture AIRTHERMY-ATX Air-Therm Heater 120 V, U.S. AIRTHERMZ-ATX Air-Therm Heater 240 V Specify line cord 2 pieces of 4.5 ft clear, coil-reinforced heater hose included with Air-Therm. Specify Line (Mains) Voltage OPTIONAL ACCESSORIES 15590 Clear hose, 2.5” diam., 4.5 ft 300276 Replacement platinum temperature probe 3491 5 ft (1.5m) probe extension cable with media from the incubator directly into the (Air-Therm controlled) recording chamber. This is a comfort level that hitherto was unattainable by scientists and clinical researchers since cells will have to be transported from the culture dishes on to cover slips just prior to imagery or attaching patch electrodes. Chamber: The most problematic aspect of any live cell imaging procedure or patch clamp approach is the thermal drift of high magnification lenses caused by temperature fluctuations in the microscope's environment. It is necessary to constantly readjust the focus during image acquisition due to this thermal-drift, introducing artifacts during quantitative microscopy due to the photo-bleaching that occurs during refocusing. The optimal solution to this problem is to use WPI’s AirTherm temperature controller connected to a Plexiglas or acrylic chamber that encloses the entire imaging area (or at least the objectives and the stage). Using such a chamber will result in highly stable temperature control and minimal thermal drift. AIR-THERM SPECIFICATIONS AIR FLOW RATE CONTROL TEMP. RANGE TEMPERATURE RESOLUTION TEMPERATURE ACCURACY CONTROL MODES ANALOG OUTPUT FOR CHART RECORDER HEATING VOLUME POWER (CE) DIMENSIONS SHIPPING WEIGHT 20 to 50 CFM (0.55 to 1.4 cubic meter/M) Ambient to 60°C 0.1 °C 0.2 °C (a) Auto (PID set manually, or Self-Tune) (b) Manual tune 0.5°C resolution 0-10V, 0 to 100 C (default, adjustable via controller) More than 4 cu ft (85 liter), non-circulating 300 W, 95-135 V or 220-240 V, 50/60 Hz 61⁄2 x 8 x 71⁄2 in. (15.5 x 21 x 19 cm) 10 lb (4.5 kg) 160 World Precision Instruments UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com