EPITH ELIAL PHYS IOLOGY Automated TEER Measuring System The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a PC-controlled, tissue resistance measurement system that offers reproducibility, accuracy, flexibility and ease-of-operation for this kind of measurement. Automated measurement of tissue resistance in cell culture microplates provides the important advantages of speed, precision, decreased opportunity for contamination and the instant availability of measured resistance data on a computer. These measurements are useful in applications such as drug bioavailability studies and studies on the mechanisms of drug transport. The main components of the REMS AutoSampler include: the robotic sampler that moves the electrode over each well of the microplate, the electrode which is located on the robotic arm, a base plate for the 24- and 96-well tray, a Windows-based data acquisition card, the REMS interface unit and the REMS software to operate the system on a Windows-based computer. The REMS AutoSampler automates TEER measurements previously made with WPI’s EVOM Epithelial Voltohmmeter. Automated tissue resistance measurements up to 20 kΩ can be performed on 24- or 96-well HTS microplates. Microplates presently supported include the Corning Costar HTS Transwell-24, Falcon HTS Multiwell insert systems, and Millipore Multiscreen™ CaCo 96-well plate. REMS AUTOSAMPLER SPECIFICATIONS MEMBRANE RESISTANCE RANgE AC SqUARE WAVE CURRENT ElECTRODE POSITIONINg ElECTRODE PERFORMANCE ElECTRODE ARM SPEED TyPICAl MEASUREMENT TIME 24-WEll SCAN PATTERN lINE VOlTAgE DIMENSIONS WEIgHT 0 to 2000 Ω and 0 to 20 kΩ +/- 20 µA @ 12.5 Hz Resolution in X, y and Z: +/- 1 mm Repeatability in X, y and Z: +/- 0.25 mm X- and y-axis: 250 mm/sec Z-axis: 247.3 mm/sec 1 min, 10 sec Choice of any well pattern sampling User specified: 100/120 V or 220/240 V 53.5 × 43.7 × 37.1 cm (213⁄32 × 173⁄16 × 145⁄8 in.) 24 kg (52 lb) to reproducibly and precisely locate the electrode results in highly reproducible TEER measurements. TEER measurements are stored in the computer as the electrode moves from one well to the next. The Windows-based software provides user-friendly features to acquire, display and store the tissue resistance measurements. The REMS AutoSampler is designed to facilitate integration with The REMS electrode is very compact and robust in design. Each of two other robotic systems. Special locating bars are installed on the REMS rod-shaped probes, 1.5 mm in diameter, consists of a pair of electrodes: base platform that allow other system robots to place an HTS tray into a one electrode for injecting current and the other for measuring the precise location on the REMS base. voltage. The use of two pairs of electrodes eliminates the error caused The REMS AutoSampler will automatically measure and record by the electrode-liquid interface. To take a measurement, the robot tissue resistance from a user-specified matrix of culture wells on the inserts one probe into the center of the filter well and the other into the microplate. According to the specified sequence, the robotic arm moves opening slot of the 24- or 96-well plate. The use of AC current to measure over the identified wells taking TEER measurements. By means of a x-y-z resistance provides several advantages over DC current, including: locating system, the electrode-containing arm is positioned precisely ● Absence of offset voltages on measurements; and reproducibly over each well. The ability of the REMS AutoSampler ● There is a zero net current being passed through the membrane and, therefore, it is not adversely affected by a REMS Automated Tissue Resistance Measuring System current charge; Includes robot sampler, base plate, data acquisition board; computer, display, ● No electrochemical deposition of electrode keyboard, mouse; software for Windows XP or Vista; and electrode for either metal. 24-well plate (Corning Costar HTS Transwell-24 or Falcon HTS Multiwell) or 96-well The REMS AutoSampler also features a rinse plate (Millipore Multiscreen CaCo) — SPECIFY WHEN ORDERING. and calibration check station. If occasional ACCESSORIES rinsing of the REMS electrode is required it may REMS-24 Replacement REMS STX Electrode for 24-well HTS Plate be sent to a rinse station by pressing the rinse REMS-96 Replacement REMS STX Electrode for MilliporeTM 96-well Plate station button on the menu bar. Contact WPI for detailed information. World Precision Instruments www.wpiinc.com 16 UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com