Langendorff M U SCLE PHYS IOLOGY Isolated perfused heart system The SI-LAnG1 and SI-LAnG2 isolated perfused heart systems were developed for in vitro investigations of the hearts of small animals according to the methods originated by Oscar Langendorff . The system can be configured easily to perform many of the perfusion techniquesused to study isolated hearts . Supplementary devices, like a special MAP sensor or pacing/recording electrodes, can easily be added to the system . Isolated Organ Features l Retrograde perfusion of isolated hearts from mouse, rat, guinea pig, hamster and rabbit l Measurements in constant pressure or constant flow mode on the same device l Can be equipped with two columns for using different buffers l Capable of recording aortic pressures as high as 120mmHg l Equipped for measuring left ventricular pressure (LVP) l Ports in the system are available for measuring pressure, flow, temperature and surface potentials, and for the infusion or injection of drugs l Continuous filling of buffer columns with use of a pump l Continuous oxygenation of buffers l Water-jacketed system for precise temperature control The Langendorff system is designed as a perfusion system for isolated, small mammalian hearts. Some special features of this system include the stainless steel sink, the small reeling pump and specially designed two-way Teflon taps. LANGENDORF SYSTEMS SI-LANG1 Langendorf Isolated Perfused Heart System, Single Buffer Column SI-LANG2 Langendorf Isolated Perfused Heart System, Dual Buffer Column Included: Table with base plate, shelf, sink • Console • Perfusion buffer column • Glass overflow tube Teflon tap set • Heart suspension unit • Heart chamber • Spindle syringe • Pressure sensor holders (2) • Oxygenation bubbler set • Oxygenation pressure equalizer set • Latex pressure balloons for left ventricular pressure (LVP) catheter • PeriStar Pro 2-channel peristaltic pumps (2) • Circulating water bath • Pressure transducers with cables (2) OPTIONAL COMPONENTS SI-SEN12AM4E-U Manipulator with Removable Head (Unipolar) SI-SEN12AM4E-B Manipulator with Removable Head (Bipolar) SI-SEN07RTH2 Buffer Temperature Sensor SI-SEN13MAP Monopolar Action Potential Sensor The Isolated Perfused Organ (IPO) system is designed as a perfusion system for isolated, small mammalian organs like the kidney, liver or mesenteric bed. The SI-IPOKIDnEY system is designed for in vitro investigations of the perfused kidney of small laboratory animals like mice, rats, hamsters, guinea pigs and rabbits . An optional perfusion chamber for liver and mesenteric bed (gut) is also available . During experiments, the artery of the organ is cannulated and perfused with buffer solutions using either constant pressure or constant flow mode . Then, you can measure the changes in the perfusion pressure . If desired, the effluents can be collected for biochemical analysis . 8 World Precision Instruments UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com