Screening (HTS) cell culture filter plates EPITH ELIAL PHYS IOLOGY Also for use with Millicell ERS-2 Endohm™ For TEER measurement of endothelial cell cultures in individual cups ● Compatible with EVOM2 ● Improved accuracy of 1-2 Ohm ● Accommodates 6mm, 12mm, 24mm cups and Costar Snapwell cup ● Sterilized with EtO, alcohol or a bactericide held STX2 electrodes) to less than 5 Ω. With Endohm’s fixed electrode geometry, variation of readings on a given sample is reduced from 10-30 Ω with STX2 electrodes (depending on the experience of the user) to 1-2. Compared with other resistance measurement methods, Endohm with EVOM2 offers a much more convenient and economic solution to “leaky tissue” measurement. Because of the uniform density of the AC square wave current from EVOM2, errors owing to electrode polarization or membrane capacitance are largely eliminated. Endohm together with EVOM2 offers the most accurate and economical endothelial ohmmeter now available. To date, cups from Costar, Millipore, ICN Biomedicals, and Falcon have been tested. Endohm chambers may be sterilized with EtO, alcohol or a bactericide (also see: Cidex, Microsurgery section); not autoclavable. Using WPI’s EVOM2 resistance meter, Endohm chambers provide reproducible resistance measurements of endothelial tissue in culture cups. Transfer cups from their culture wells to the Endohm chamber for measurement rather than using hand-held electrodes. The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. The height of the top electrode can be adjusted to fit cell culture cups of different ENDOHM-6 Endohm for 6 mm culture cup (24 wells per plate) manufacture. Endohm’s symmetrically apposing circular disc ENDOHM-12 Endohm for 12 mm culture cup (12 wells per plate) electrodes, situated above and beneath the membrane, allow ENDOHM-24SNAP Endohm for 24 mm & Costar Snapwell™ cup (6 wells per plate) a more uniform current density to flow across the membrane Requires EVOM2, EVOM or EVOMX to operate than with STX2 electrodes. The background resistance of a 53330-01 Replacement Endohm Cable blank insert is reduced from 150 Ω (when using WPI’s handWorld Precision Instruments UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com 15