Current (pA) – unique flexible NO sensor! Designed for arteries, microvessels, in vivo applications, and similar applications. ISO-NOPF electrodes are the newest addition to WPI’s nitric oxide sensor family and are available in 100 µm and 200 µm diameters. Utlilzing the latest advances in nanotechnology and material science, scientists at WPI’s Sensor Laboratory have created these completely flexible and virtually unbreakable NO sensors. The new sensors are based on a composite graphite NO-sensing element combined with a reference electrode. The surface of the sensor is then coated with a unique multi-layered NO-selective membrane. iSo-nopF 50 pA Sensor Body 60mm x < 2mm∅ 10 sec 4 nM 2 nM 8 nM Ag/AgCl flexible 100, 200 or 500µm 1 nM 5mm 2mm Time (s) Response of ISO-NOPF to NO. Schematic drawing of ISO-NOPF. iSo-nopF100 iSo-nopF200 iSo-nopF500 91580 Snap50 100 µm Flexible NO Sensor, pkg of 2 200 µm Flexible NO Sensor, pkg of 2 500 µm Flexible NO Sensor, pkg of 2 Microsensor Adapter Cable SNAP, 50 mg vial microns and is available in two different tip lengths (i.e., ISO-NOP3020 has tip length of 2 mm, ISO-NO3005 has tip length of 0.5 mm). The response of the ISO-NOP007 and ISO-NOP30 is linear over a wide dynamic concentration range of NO. The design of both electrodes is based on a single carbon fiber coated with WPI’s NO-selective membrane. A detection limit of approximately 1 nM NO makes these electrodes ideal for use in tissues and microvessels. iSo-nop007 iSo-nop30 – 7 and 30 micron sensors with exceptional performance — ideal for tissues and microvessels The ISO-NOP007 and ISO-NOP30 have recently been improved in design and performance. The ISO-NOP007 has a tip diameter of just 7 microns and a length of 2 mm. The ISO-NOP30 has a tip diameter of 30 BIOS EN S I NG Current (pA) 300 pA 20 sec 1600 Sensor Body Ag/AgCl 60 mm x < 2 mm diam. 2 mm or 0.5 mm Current / pA 1200 y = 1.3583x + 16.15 R2 = 0.9985 800 7 µm or 30 µm 400 0 0 300 600 900 1200 Concentration / nM Time (s) 2 mm Schematic drawing of ISO-NOP007 and ISONOP30. The response of a 7 µm NO sensor (ISO-NOP007) to successive additions of NO (100 nM). Inset shows the linearity of the resulting calibration plot. iSo-nop007 iSo-nop3020 iSo-nop3005 91580 Snap50 7 µm Nitric Oxide Sensor (pkg of 3) 30 µm Sensor Tips (2 mm length), pkg of 3 (requires #91580) 30 µm Sensor Tips (0.5 mm fiber), pkg of 3 (requires #91580) Microsensor Adapter Cable SNAP, 50 mg vial Simultaneous measurement of force (top trace) and changes of NO concentration (lower trace) in (A) the rat superior mesenteric artery relaxed with ACh and (B) a small human artery relaxed with ACh and SNAP. In this artery oxyhaemoglobin(oxyHb) partly reversed the increase in NO concentration, with only a small change in force. [U. Simonsen, et al., J. Physiol., 1999, 516: 271-282.] GLucoSe SenSor SpeciFicationS IN VITRO PRECISION GLUCOSE RANGE RESPONSE TIME (sec) IN VIVO CALIBRATION INTERFERENCE SPECIES LENGTH SENSOR SIzE REFERENCE ELECTRODe POLARIzATION VOLTAGE (V) SENSOR LIFE Coefficient of Variation (CV) ≤5% 36 - 450 mg/dl (or 2-25 mM/L) 100 - 300s In vivo calibration Acetaminophen, ascorbic acid, uric acid 5 cm 0.6 × 0.7 mm Ag/AgCl 0.65 -0.7V vs. Ag/AgCl 3-4 months in solutions at room temperature under continuous polarization; 15-30 days in vivo 6 months 20° to 40° C (68° to 104° F) 10° to 25° C (50° to 77° F) iGS100 – Implantable glucose sensor Measuring glucose in vivo over the long term is challenging and difficult. Previous measurement systems were limited to acute studies or a few days at best. WPI introduces a new kind of implantable glucose sensor based on a patented technology. This sensor provides a tool for researchers to directly detect glucose in chronic studies in vitro or in vivo. The sensor is fully compatible with WPI’s Apollo system. iGS100 91580 Implantable Glucose Sensor (pkg of 2) Microsensor Adapter Cable SHELF LIFE OPERATION CONDITIONS STORAGE CONDITIONS World Precision Instruments UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com 55