Ordering an Environmental Chamber Acrylic enclosures are essentially custom-built. When ordering a system, you will need to provide the following information: Microscope Perfect Focus Right Port Tirf Tilting Head Emission Cells Stage Camera Analyzer White Light Tirf Filter Wheels: Dual Lamphouse 35mm/60mm Coverslips Stage-Up Left Port Fluor Attachment Binocular D Head Excitation Transmitted Light Shutter Wells Consistent Air Flow Air flow affects the temperature uniformity of incubators. The red arrows on Fig. 1 and Fig. 3 indicate air flow. The Live Cell Microscope Incubator uses a diffuser grid and proper venting to insure consistent air flow. Traditional incubators with poor air flow suffer with hot and cold spots in the incubator, as seen in thermal images (Fig. 2 and Fig. 4). Warmer temperatures are indicated by red and cooler temperatures by blue. MICROSCOPES, CAMERAS Fig. 1–Single air input and no venting causes random air flow in a traditional incubator. Fig. 2–Hot and cold spots result from inconsistent flow. HOC adds control of the O2 level, which is accomplished by displacing the oxygen with nitrogen. The first three ECU units are capable of controlling a simple, external heater, like the AirTherm Satelite (AirTherm-SAT) or a microscope lens warmer. The AirTherm-SMT can monitor and control both temperature and humidity inside the microscope chamber. IV-100SMT IV-200ECU Fig. 3–A diffusion grid with air input and exhaust vents yields consistent air flow. Fig. 4–Consistent air flow means uniform heating. Environmental Chamber and AirTherm-SMT. Incubation system with flow regulator, proprietary humidification module, stage adapter, stage dish, optical grade glass. System utilizes premixed 5% CO2 gas cylinders. Environmental Chamber with CO2 and heat regulated via ECU-HC and AirTherm-SAT. Incubation system, proprietary humidification module, stage adapter, stage dish, optical grade glass. System requires 100% CO2 and ambient air gas supply and mixes at a defined rate. Environmental Chamber actively regulated CO2 and heat via ECU-HCP and AirTherm-SAT. Incubation system, proprietary humidification module, stage adapter, stage dish, optical grade glass. System probe directly measures CO2 concentration at the sample and adjusts the stream of incoming CO2 via feedback. System requires 100% CO2 and ambient air gas supply and mixes at a defined rate. Environmental Chamber with O2, CO2 and heat via ECU-HOC and AirTherm-SAT. Incubation system, proprietary humidification module, stage adapter, stage dish, optical grade glass. System requires 100% CO2 and ambient air gas supply and mixes at a defined rate. Custom Design Fee — includes (but not limited to) Tilting Heads, Black Chamber, Confocals, Camera on Right, HMX Lamphouse, and Manipulators. World Precision Instruments www.wpiinc.com Germany: Tel: +49 (0)30-6188845 • wpide@wpi-europe.com IV-300ECU IV-200-OX IV-CUST UK: Tel: +44 (0)1462 424700 • wpiuk@wpi-europe.com US: Tel: 941-371-1003 • sales@wpiinc.com 157