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>
Don't Mask Ultra-High Performance
Tips and Tricks for Using Core-shell Columns
on HPLC Instruments
Minimize the extra-column volume
from the injector to the column
· Use a low-volume injection system if you are not using an autosampler
· Use 0.12 mm ID (0.005 in.) or 0.17 mm ID (0.007 in.) tubing whenever possible
· Minimize the length of all connection tubing
· Ensure tubing is seated properly at every connection
· Use extremely low dead-volume fittings (see page 53)
· Use an extremely low dead-volume in-line filter
(see SecurityGuard
TM
ULTRA on pages 36, 37, 53, and 55)
Minimize peak dispersion and detector
contribution after the column
· To avoid extra column band broadening effects, check the flow cell volume to verify
that it is no more than
1
/
3
the peak volume
· Standard flow cells on conventional LC systems can be > 10 µL. For best results,
replace standard flow cells with < 3 µL flow cells (< 2 µL when using 2.1 mm ID
columns).
· Increase the detector scan rate. 0.1 seconds to 0.15 seconds is recommended for
increased data collection.
Maximize Performance
with Kinetex 2.6 µm
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enhancement
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