42
>
C8
A
pp ID 19465
C18
A
pp ID 19466
Complementary Selectivities
With complementary reversed phases selectivities you can screen for optimal resolution of
complex sample mixtures. Manipulate acidic and basic compound elution by simply switch-
ing between column chemistries.
Conditions for both columns:
Column: Kinetex
®
2.6 µm XB-C18
Kinetex 2.6 µm C18
Dimensions: 50 x 2.1 mm
Mobile Phase: A: 0.1% Formic acid in Water
B: 0.1% Formic acid in Acetonitrile
Gradient: Time (min)
0.0
0.2
4.2
4.21
5.5
% B
5
5
95
5
5
Flow Rate: 0.8 mL/min
Temperature: 30 ºC
Detection: UV @ 254 nm (ambient)
Sample: 1. Pyridine
2. Acetaminophen
3. Pindolol
4. Quinidine
5. Sulfathiazole
6. Acebutolol
7. Benzyl alcohol
8. Chlorpheniramine
9. Phenol
10. Triprolidine
11. Nortriptyline
12. Prednisolone
13. 3-Methyl-
4-nitrobenzoic acid
14. 2-Hydroxy-
5-methylbenzaldehyde
15. Diflunisal
16. Hexanophenone
Increase resolution between compounds
Comparative separations may not be representative of all applications.
4
5
1
7
2
5
3
8
4
6
6
19465
7
min
4
19466
1
2
7
5
3
4,8
6
5
6
7
min
Conditions for both columns:
Column: Kinetex 2.6 µm C8
Kinetex 2.6 µm C18
Dimensions: 100 x 4.6 mm
Mobile Phase: A: 0.1% Phosphoric acid in Water
B: 0.1% Phosporic acid in Acetonitrile
Gradient: Time (min)
% B
0
30
4
50
7.5
70
Time (min)
% B
9
95
10
30
Flow Rate: 1.5 mL/min
Temperature: 25 ºC
Detection: UV @ 230 nm
Sample: 1. Naproxen
2. Fenoprofen
3. Ibuprofen
4. Phenylbutazone
5. Indomethacin
6. Meclofenamic acid
7. Matrix impurity
8. Matrix impurity
Change your peak elution profile
App ID 22076
App ID 22075
C18
22076
0
1
2
3
4+5
8
7
6
9
10
11
12
13
14
15
16
1
2
3
min
XB-C18
22075
0
1
2
3
4+5
6
7
8
9
10
11
12
13
14
15
16
1
2
3
min
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