46
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25-OH Vitamin D2
and D3 from Serum
Ethyl Sulfate and Ethyl Glucuronide
Testosterone from Male Plasma
Wide Applicability Across Many Industries
For Clinical Analysis
Column: Kinetex
®
2.6 µm C18
Dimensions: 50 x 4.6 mm
Part No.: 00B-4462-E0
Mobile Phase: A: 0.05 % Formic acid
B: 5 mM Ammonium acetate + 0.1 % Formic acid in Methanol
Gradient:
Time (sec)
0
5
205
290
360
% B
8
8
100
100
8
Flow Rate: 1 mL/min
Temperature: 35 ºC
Detection: MS (ambient)
Sample : 1. 25-hydroxyvitamin D3 (25-OH D3)
2. 25-hydroxyvitamin D3-d6 (25-OH D3-d6)
3. 25-hydroxyvitamin D2 (25-OH D2)
Column: Kinetex 2.6 µm C18
Dimensions: 100 x 2.1 mm
Part No.: 00D-4462-AN
Mobile Phase: A: 5 mM Ammonium formate
B: Methanol
Gradient: Time (min)
0
3
4
4.01
% B
5
10
95
5
Flow Rate: 0.2 mL/min
Temperature: 25 ºC
Detection: MS (ambient)
Sample : 1. Ethyl Glucuronide (EtG) + IS (EtG-d5)
2. Ethyl Sulfate (EtS) + IS (EtS-d5)
Column: Kinetex 1.7 µm C18
Dimensions: 30 x 2.1 mm
Part No.: 00A-4475-AN
Mobile Phase: A: 0.1 % Formic acid + 1 mM Ammonium Formate in Water
B: 0.1 % Formic acid + 1 mM Ammonium Formate in Acetonitrile
Gradient: Time (min)
0
2.5
3.5
3.6
% B
10
90
90
10
Flow Rate: 0.4 mL/min
Temperature: 55 ºC
Detection: MS (ambient)
Sample : 1. Testosterone
2. Testosterone-d3
25-OH D3-d6 (IS)
25-OH D2
25-OH D3
19313
3.6 3.7 3.8 3.9 4.0 4.1 4.2 4.3 4.4 4.5 4.6min
4.0e4
3.0e4
2.0e4
1.0e4
5.0e4
6.0e4
0
Intensity cps
App ID 19313
1
19028
2
0.5
0
1
1.5
2
2.5
min
5000
1.0e4
1.5e4
2.0e4
Intensity
, cps
App ID 19028
Intensity cps
Intensity cps
Intensity cps
19760
1
2
Intensity
, cps
0.0
5000.0
1.0e4
1.5e4
2.0e4
2.5e4
3.0e4
3.5e4
4.0e4
4.5e4
5.0e4
5.5e4
6.0e4
6.5e4
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5 min
App ID 19760
Phenomenex
l
WEB: www.phenomenex.com