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0
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10
12
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10
mAU
18827
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Waters
®
Symmetry
®
5 µm C18
Decrease Column Length
**
64%
Higher Throughput than
Original EP Method
Dimensions: 150 x 3.9 mm
Mobile Phase: 12.5 mM Phosphoric acid in Water, pH 3.0 + 2.0 g Sodium
octanesulfonate + 0.8 g Tetrabutyl ammonium hydrogen
sulfate / Methanol / THF (80:18:2)
Flow Rate: 0.6 mL/min
Temperature: 22 ºC
Detection: UV @ 226 nm
Sample: Atenolol Related Substance
1. Impurity B
2. Impurity A
3. Impurity J
4. Impurity I
5. Impurities D and E
6. Impurity F
7. Impurity G
8. Impurity H
* European Pharmacopeia (Ph. Eur.)
Waters and Symmetry are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Waters Corporation.
Comparative separations may not be representative of all applications.
* Decrease in column particle within allowable Ph. Eur. and USP pharma particles size change (+- 50 %)
**
Decrease in column length within allowable Ph. Eur. and USP column length change (+- 70 %)
Replace 3 µm and 5 µm Columns.
For Improved Speed, Resolution, and Sensitivity
Generating much lower backpressure (< 400 bar) at optimal linear velocities, you can now
achieve 2-3x's the column efficiencies of traditional fully porous 3 µm and 5 µm columns on
any LC instrument.
*Ph. Eur. Specified 5 µm C18 Column
18514
mAU
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min
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15
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20
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App ID 18514
App ID 18827
18514
4
Column: Kinetex 2.6 µm C18
Dimensions: 100 x 4.6 mm
Part No.: 00D-4462-E0
Mobile Phase: 12.5 mM Phosphoric acid in Water, pH 3.0
+ 2.0 g Sodium octanesulfonate + 0.8 g
Tetrabutyl ammonium hydrogen sulfate /
Methanol / THF (80:18:2)
Flow Rate: 0.9 mL/min
Temperature: 22 ºC
Detection: UV @ 226 nm
Sample: Atenolol Related Substance
1. Impurity B
2. Impurity A
3. Impurity J
4. Impurity I
5. Impurities D and E
6. Impurity F
7. Impurity G
8. Impurity H
4
3
Phenomenex
l
WEB: www.phenomenex.com