18
>
Conditions for both columns:
Columns: Kinetex 5 m C18
NUCLEOSIL 5 µm C18
Dimension: 150 x 4.6 mm
Mobile Phase: A: Milli-Q
®
Water
B: Acetonitrile
Gradient: Time (min)
0
15
16
17
20
% B
30
95
95
30
30
Flow Rate: 1.5 mL/min
Temperature: Ambient
Detection: UV @ 205 nm
Sample: 1. 6-Gingerol (1)
2. 6-Gingerol (2)
3. Capsaicin
4. 8-Gingerol
5. 6-Shogaol
6. 10-Gingerol
7. 8-Shogaol
8. 10-Shogaol
NUCLEOSIL is a registered trademark of Macherey Nagel. Milli-Q is a registered trademark of Merck KGaA, Darmstadt, Germany.
Phenomenex is not affiliated with Macherey Nagel or Merck KGaA. Comparative separations may not be representative of all applications.
Macherey Nagel NUCLEOSIL
®
5 µm C18
Kinetex
®
5 µm C18
20773
1
2
3
4
5
6
7
8
0
10
20 min
0
20
40
60
80
100
mAU
1
2
3
4
5
6
7
8
0
10
20 min
0
20
40
60
80
100
mAU
20772
App ID 20772
App ID 20773
193 Bar
213 Bar
90% Increase
in sensitivity
Enhanced Sensitivity
without Pressure Increase
Kinetex 5 µm
core-shell columns easily provide enhanced sensitivity on any HPLC system
without an increase in backpressure.
TM
5
µm
Phenomenex
l
WEB: www.phenomenex.com