48
>
Forced Degradation
of Ranitidine
Tricyclic Antidepressants
Pharmaceutical Mixture
Wide Applicability Across Many Industries
For Pharmaceutical Analysis
App ID 18600
Column: Kinetex
®
2.6 µm C18
Dimensions: 150 x 4.6 mm
Part No.: 00F-4462-E0
Mobile Phase: A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in Acetonitrile
Gradient: 5 % to 20 % B in 7 min. 20 % to 95 % in 2 min
Flow Rate: 1.4 mL/min
Temperature: 30 ºC
Detection: UV @ 230 nm (22 ºC)
Sample: Ranitidine 1 mg/mL in Methanol. Heated at 65 ºC for 4 days.
Column: Kinetex 1.7 µm C18
Dimensions: 50 x 2.1 mm
Part No.: 00B-4475-AN
Mobile Phase: A: Water with 0.05 % Formic acid
B: Acetonitrile with 0.05 % Formic acid
Gradient: Time (min)
0
2
3
3.1
4.5
% B
30
40
95
30
30
Flow Rate: 0.6 mL/min
Temperature: 40 ºC
Detection: MS (ambient)
Sample : 1. Doxepin
2. Desipramine
3. Imipramine
4. Nortriptyline
5. Amitriptyline
6. Trimipramine
Column: Kinetex 2.6 µm C8
Dimensions: 50 x 2.1 mm
Part No.: 00B-4497-AN
Mobile Phase: A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in Acetonitrile
Gradient: Time (min)
0
0.2
4
% B
5
5
95
Flow Rate: 0.8 mL/min
Temperature: 22 ºC
Detection: UV @ 254 nm (ambient)
Sample : 1. Pyridine
2. Acetaminophen
3. Sulfathiazole
4. Pindolol
5. Quinidine
6. Benzyl Alcohol
7. Phenol
8. Acebutolol
9. Chlorpheniramine
10. Triprolidine
11. Prednisolone
12. 3-Methyl-4-nitrobenzoic acid
13. Nortriptyline
14. 2-Hydroxy-5-methylbenzaldehyde
15. Diflunisal
16. Hexanophenone
0
2
4
6
8
10
12
mAU
0
1
2
3 min
16
19476
15
14
12
13
11
9
10
8
7
6
4,5
3
2
1
App ID 19476
1
19306
2
3
4
5
6
1
2
min
App ID 19306
0
18600
2
4
6
min
0
20
40
60
mAU
80
100
Degradation
Products
Degradation Products
0
18599
2
4
6
min
App ID 18599
Undegraded Sample
Phenomenex
l
WEB: www.phenomenex.com