30
>
Kinetex
®
1.7 m for Higher Resolution
Do everything you can to make sure you are achieving the resolution needed by using a
powerful Kinetex Core-Shell sub-2 µm column.
TM
1.7
µm
AppID19804
AU
1 2
3
4
5
6
7
8
min
1.6
1.8
2.0
2.2
2.4
2.6
2.8
3.0
3.2
3.4
3.6
3.8
4.0
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
Waters, ACQUITY, and UPLC are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Milli-Q is a registered trademark of Merck KGaA,
Darmstadt, Germany. Phenomenex is not affiliated with Waters Corporation or Merck KGaA. Comparative separations may not be representative of all applications.
Waters
®
ACQUITY
®
BEHTM 1.7
µm
Phenomenex
®
Kinetex 1.7
µm
Conditions for both columns:
Column: Kinetex 1.7 µm XB-C18
ACQUITY UPLC BEH 1.7 µm C18
Dimensions: 150 x 2.1 mm
Mobile Phase: A: Milli-Q
®
Water
B: Acetonitrile
Gradient: Time (min)
0
5
% B
30
100
Flow Rate: 0.5 mL/min
Temperature: Ambient
Detection: UV @ 210 nm (ambient)
Backpressure: 981 bar (Kinetex)
935 bar (ACQUITY)
Instrument: Waters ACQUITY UPLC
Sample: 1. Hydrocortisone
2. Cortisone
3. Corticosterone
4. Cortisone-21-Acetate
5. DHEA (Dehydroepiandrosterone)
6. 17-Hydroxprogesterone
7. Deoxycorticosterone
8. Progesterone
Column
Resolution between
Hydrocortisone and
Cortisone
Resolution between DHEA
(Dehydroepiandrosterone)
and 17-Hydroxyprogesterone
Resolution between
Deoxycorticosterone and
Progesterone
Kinetex 1.7 µm
XB-C18 150 x 2.1 mm
2.5
(66 % increase)
5.0
(138 % increase)
7.4
(21 % increase)
ACquITy
®
1.7 µm
BEH
TM
C18 150 x 2.1 mm
1.5
2.1
6.1
1
2
3
4
5
6
7
8
AppID19805
AU
min
1.6
1.8
2.0
2.2
2.4
2.6
2.8
3.0
3.2
3.4
3.6
3.8
4.0
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
App ID 19805
App ID 19804
66 %
Higher Resolution
(between peaks 1 and 2)
138 %
Higher Resolution
(between peaks 5 and 6)
21 %
Higher Resolution
(between peaks 7 and 8)
Phenomenex
l
WEB: www.phenomenex.com