43
>
1
18605
2
3
min
2
1
Orthogonal Chemistries
Whether you are looking for a confirmation column or an orthogonal selectivity, both the
Kinetex Phenyl-Hexyl and HILIC stationary phases offer excellent solutions for you.
Norepinephrine and Epinephrine
App ID 18605
App ID 18604
HILIC
Conditions for both columns:
Columns: Kinetex 2.6 µm HILIC
Kinetex 2.6 µm C18
Dimensions: 50 x 2.1 mm
Mobile Phase (HILIC): Acetonitrile / 100 mM Ammonium formate
pH 3.2 (92:8)
Mobile Phase (C18): 5 mM Ammonium formate pH 3.2 /
Methanol (97:3)
Flow Rate: 0.4 mL/min
Temperature: 30 °C
Detection: UV @ 210 nm (ambient)
Sample: 1. Norepinephrine
2. Epinephrine
18604
1
2
3
min
1
2
C18
Comparative separations may not be representative of all applications.
Conditions for both columns:
Column: Kinetex 2.6 m C18
Kinetex 2.6 m Phenyl-Hexyl
Dimensions: 50 x 2.1 mm
Mobile Phase: A: 0.1% Formic acid in Water
B: 0.1% Formic acid in Acetonitrile
Gradient: Time (min)
0.0
0.2
4.2
4.21
5.5
% B
5
5
95
5
5
Flow Rate: 0.8 mL/min
Temperature: 30 ºC
Detection: UV @ 254 nm (ambient)
Sample: 1. Pyridine
2. Acetaminophen
3. Sulfathiazole
4. Pindolol
5. Quinidine
6. Benzyl alcohol
7. Phenol
8. Acebutolol
9. Chlorpheniramine
10. Triprolidine
11. Prednisolone
12. 3-Methyl-4-nitrobenzoic acid
13. Nortriptyline
14. 2-Hydroxy-5-methylbenzaldehyde
15. Diflunisal
16. Hexanophenone
App ID 22078
Phenyl-Hexyl
App ID 22075
22075
0
1
2
3
4+5
6
7
8
9
10
11
12
13
14
15
16
1
2
3
min
C18
22078
0
1
2
3
4
8
7
6
5
9
10
11
12
13
14
15
16
1
2
3
min
Phenomenex
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