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Polyaromatic Hydrocarbons (PAHs):
EPA Method 610
Estrone, Estradiol,
and Estriol from Water
Wide Applicability Across Many Industries
For Environmental Analysis
Column: Kinetex 2.6 µm C18
Dimensions: 100 x 4.6 mm
Part No.: 00D-4462-E0
Mobile Phase: A: Water
B: Acetonitrile
Gradient: (30:70) A/B to (0:100) A/B over 10 min
Flow Rate: 1.5 mL/min
Temperature: 30 °C
Detection: UV @ 254 nm
Sample : 1. Naphthalene
2. Acenaphthylene
3. Fluorene
4. Acenaphthene
5. Phenanthrene
6. Anthracene
7. Fluoranthene
8. Pyrene
9. Chrysene
10. Benz[a]anthracene
11. Benzo[b]fluoranthene
12. Benzo[k]fluoranthene
13. Benzo[a]pyrene
14. Dibenz[a,h]anthracene
15. Indeno[1,2,3-cd]pyrene
16. Benzo[g,h,i]perylene
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18552
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6
min
App ID 18552
0
0
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140
mAU
19680
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4
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1
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min
App ID 19680
Column: Kinetex 2.6 µm XB-C18
Dimensions: 150 x 4.6 mm
Part No.: 00F-4496-E0
Mobile Phase: A: 6.9 mM Ammonium acetate pH 4.0 with 25 % Acetonitrile (v/v)
B: Acetonitrile
Gradient: Time (min)
0
10
% B
0
65
Flow Rate: 1.2 mL/min
Temperature: 22 ºC
Detection: UV @ 230 nm
Sample : 1. Estriol
2. Ethyl Paraben (ISS)
3. 17B Estradiol
4. Estrone
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