47
>
Column: Kinetex 2.6 µm C18
Dimensions: 50 x 2.1 mm
Part No.: 00B-4462-AN
Mobile Phase: A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in Acetonitrile
Gradient: Time (min)
% B
0
20
2.5
80
2.51
95
Time (min)
% B
2.8
95
2.81
20
4.6
20
Flow Rate: 450 µL/min
Temperature: 25 °C
Detection: MS (ambient)
Sample: 1. Triamcinolone
2. Prednisolone
3. Cortisone
4. Betamethasone
5. Corticosterone
6. Triamcinolone acetonide
7. 11-a-Hydroxyprogesterone
8. Cortisone acetate
9. Testosterone
10. 11-Ketoprogesterone
11. Betamethasone 17-valerate
Column: Kinetex 2.6 µm C18
Dimensions: 50 x 2.1 mm
Part No.: 00B-4497-AN
Mobile Phase: A: 10 mM Ammonium acetate
B: 10 mM Ammonium acetate in Methanol
Gradient: Time (min)
0
2.5
2.51
5
% B
50
100
50
50
Flow Rate: 0.4 mL/min
Temperature: 30 ºC
Detection: MS @ 350 °C
Sample : Concentration 0.25 mg/mL each
1. Digoxin
2. Oleandrin (IS)
3. Digitoxin
Digoxin and Digitoxin in Plasma
Steroids
Wide Applicability Across Many Industries
For Clinical Analysis
Preview your current method
enhanced with Kinetex Core-Shell
Technology
www.phenomenex.com/tools/kinetexcalculator
Intensity cps
19691
1
2
3
0
200
400
600
800
1000
1200
1400
1600
1800
2000
2200
Intensity
, cps
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5 min
App ID 19691
18848
1
2
3
4
6
5
7
8
9
10
11
0.5
1
1.5
2
2.5
3
3.5
4
min
App ID 18848
Phenomenex
l
WEB: www.phenomenex.com