19
>
Increased Resolution
at a Fraction of the Pressure
Waters is a registered trademark and SunFire is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation.
Comparative separations may not be representative of all applications.
Waters
®
SunFire
TM
3.5 µm C18
Kinetex 5 µm C18
App ID 20771
Conditions for both columns:
Columns: Kinetex 5 m C18
SunFire 3.5 µm C18
Dimension: 50 x 2.1 mm
Mobile Phase: A: 30 mM Ammonium formate
B: Acetonitrile
Gradient: Time (min)
0
5
5.1
6
7
% B
5
60
100
100
5
Flow Rate: 0.5 mL/min
Temperature: Ambient
Detection: MS/MS (API 4000
TM
)
Sample: 1. DMIP
2. IQx
3. IQ
4. 8-MeIQ
5. 4,8-DiMe-IQx
6. 7,8-DiMe-IQx
7. Trp-P-2
8. Harman
9. Norharman
10. PhIP
11. AaC
12. MeAaC
13. MeIQ
Intensity
, cps
Intensity
, cps
20776
1
2
3
4
5
6
min
0.0
2.0e5
4.0e5
6.0e5
8.0e5
1.0e6
1.2e6
1.4e6
1.6e6
1.8e6
2.0e6
2.2e6
2.4e6
2.6e6
Intensity, cps
140 Bar
20776
1
2
3
4
5
6
min
0.0
2.0e5
4.0e5
6.0e5
8.0e5
1.0e6
1.2e6
1.4e6
1.6e6
1.8e6
2.0e6
2.2e6
2.4e6
2.6e6
Intensity, cps
5
6
App ID 20776
Intensity, cps
20771
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
5.5
min
0.0
2.0e5
4.0e5
6.0e5
8.0e5
1.0e6
1.2e6
1.4e6
1.6e6
1.8e6
2.0e6
2.2e6
2.4e6
2.6e6
Intensity, cps
20771
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
5.5
min
0.0
2.0e5
4.0e5
6.0e5
8.0e5
1.0e6
1.2e6
1.4e6
1.6e6
1.8e6
2.0e6
2.2e6
2.4e6
2.6e6
5
6
72 Bar
Alongside LC/UV analyses, the high performance and low pressure of the
Kinetex 5 µm
make it an excellent tool for LC/MS and LC/MS/MS. Pull out isobaric ions with the high ef-
ficiency of Kinetex 5 µm, all at low 5 µm backpressures that promote longer column lifetime
and less system strain.
Phenomenex
l
WEB: www.phenomenex.com