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Easy Pharmacopeia Method Optimization
Some of the greatest laboratory cost savings can be realized when an older method is
optimized to increase throughput and decrease solvent consumption. With the increased
efficiency of Kinetex core-shell technology, Ph. Eur. or USP methods can yield dramatic
performance improvements while staying within the allowable adjustments.
USP Monograph for the Assay of Ibuprofen
The monograph specifies using a 250 x 4.6 mm column packed with 5 m media containing
a C18 bonded phase under the isocratic conditions shown below. Using a Kinetex 2.6 µm
100 x 4.6 mm column, you can stay within the allowable adjustments specified in USP Gen-
eral Chapter <621>, and still maintain the system suitability of resolution between peaks of
no less than 2.5 and a tailing factor for all peaks of no more than 2.5.
Traditional 5 µm C18
Kinetex 2.6 µm XB-C18
0
2
4
6
8
10 min
0
100
mAU
1
2
3
19612
App ID 19612
Conditions for both columns:
Column: Kinetex 2.6 µm XB-C18, 100 x 4.6 mm
Traditional 5 µm C18, 250 x 4.6 mm
Mobile Phase: Acetonitrile/Water with 4 g Chloroacetic acid
adjusted to pH 3.0 with Ammonium hydroxide
(60:40)
Flow Rate: 2.0 mL/min
Temperature: 30 ºC
Detection: UV @ 254 nm
Sample: 1. Ibuprofen
2. Valerophenone
3. Ibuprofen related compound C
19613
0
100
mAU
1
2
3
0
1
2
min
App ID 19613
TM
Find more details on the
allowable adjustments for USP
and Ph. Eur. methods at:
www.phenomenex.com/kinetex/tech-
nicalresources
Comparative separations may not be representative of all applications.
Phenomenex
l
WEB: www.phenomenex.com