44
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Water Soluble Vitamins
Melamine and Cyanuric Acid
Column: Kinetex
®
2.6 µm HILIC
Dimensions: 150 x 4.6 mm
Part No.: 00F-4461-E0
Mobile Phase: A: Acetonitrile
B: 100 mM Ammonium formate, pH 3.2
C: Water
Gradient: Time (min)
% A
0
90
5
90
20
50
% B
% C
10
0
10
0
10
40
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV @ 260 nm
Sample: 1. Nicotinamide
2. PABA
3. Pyridoxine
4. Riboflavin
5. Nicotinic Acid
6. Ascorbic Acid
7. Folic Acid
8. Vitamin B12
9. Thiamine
Antibiotic Screen
Wide Applicability Across Many Industries
For Food Analysis
With complementary C18 and Phenyl phases you can screen for optimal resolution of complex sample mixtures. Manipulate
acidic and basic compound elution by simply switching between the two column chemistries.
API 3000 is a trademark of AB SCIEX.
Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation.
Phenomenex is not affiliated with Waters Corporation.
Comparative separations may not be representative of all applications.
1
2
3
4
5
6
7
8
9
0
10
20
min
0
100
200
mAU
18442
App ID 18842
App ID 18723
App ID 18555
Column: Kinetex 2.6 µm HILIC
Dimensions: 50 x 2.1 mm
Part No.: 00B-4461-AN
Mobile Phase: Acetonitrile / 100 mM Ammonium acetate, pH 5.8 (90:10)
Flow Rate: 1.0 mL/min
Temperature: 25 ºC
Detection: API 3000
TM
MS
Backpressure: 190 bar
Instrument: Waters
®
ACQUITY
®
UPLC
®
MS/MS
Sample : 1. Cyanuric acid 128-85.0
(quant ion),128.0-42.0 (qualifier ion)
2. Cyanuric acid-13C3 ISTD 131.1-87.0
3. Melamine 127.1-85 (quant ion), 127.1-68 (qualifier ion)
4. Melamine-13C3.15N3 ISTD 133.2-89.1
3,4
1,2
ESI- ESI
+
mV
18723
0.1
0.2
0.3
0.4
min
4.2e4
3.0e4
2.2e4
1.4e4
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
0
2.0e4
4.0e4
6.0e4
8.0e4
1.0e5
1.2e5
1.4e5
1.6e5
1.8e5
2.0e5
2.2e5
2.4e5
2.6e5
2.8e5
3.0e5
3.2e5
3.4e5
3.6e5
3.8e5
4.0e5
4.2e5
4.4e5
4.6e5
I
n
t
e
n
s
i
t
y
,
c
p
s
1
2
3
9
13
14
16
20, 21
22
23
24
25
26
27
28
29
30
18
10
11, 12
18555
4
5
15
19
6-8
min
Column: Kinetex 2.6 µm C18
Dimensions: 50 x 2.1 mm
Part No.: 00B-4462-AN
Mobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Methanol
Inj. Volume: 10 µL
Flow Rate: 0.5 mL/min
Temperature: 40 ºC
Detection: Mass spectrometer (MS)
Sample : 1. Sulfanilamide
2. Amoxicillin
3. Lincomycin
4. Sulfadiazine
5. Sulfathiazole
6. Ampicillin
7. Thiamphenicol
8. Sulfamerazine
9. Tetracycline
10. Ciprofloxacin
11. Enrofloxacin
12. Danofloxacin
13. Sulfamethazine
14. Sarafloxacin
15. Sulfamethoxypyridazine
16. Florfenicol
17. Spiramycin
18. Chlorotetracycline
19. Sulfadoxine
20. Clindamycin
21. Tilmicosin
22. Chloramphenicol
23. Sulfadimethoxine
24. Sulfaquinoloxaline
25. Erythromycin
26. Tylosin
27. Josamycin
28. Penicillin G
29. Cloxacillin
30. Flunixin
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l
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