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18578
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App ID 18578
Increased Sensitivity
The combination of the uniform particle shape, narrow particle size distribution, and the
significantly shorter diffusion path results in much higher column efficiencies and increased
chromatographic resolution. The increased efficiencies provide an immediate benefit in
sensitivity since higher chromatographic efficiencies translate into significantly narrower and
taller peaks, making it easier to detect low level impurities.
Signal-to-noise ratio of peak 2
Inertsil is a registered trademark of GL Sciences Inc. Phenomenex is in no way affiliated with GL Sciences Inc. Comparative separations may not be representative of all
applications.
Conditions same for both columns except where noted:
Columns: Kinetex 2.6 µm C18 100 Å
Inertsil 5 µm ODS-3 100 Å
Dimensions: Kinetex: 150 x 4.6 mm
Inertsil: 250 x 4.6 mm
Mobile Phase: A: 0.1 % Phosphoric acid in Water
B: 0.1 % Phosphoric acid in Acetonitrile
Gradient: 5 % to 95 % B in 9 min (150 x 4.6 mm)
5 % to 95 % B in 15 min (250 x 4.6 mm)
Flow Rate: 1.8 mL/min
Temperature: 50 °C
Detection: UV @ 215 nm (22 °C)
Sample: 1. Procainamide
2. Acetaminophen
3. Folic acid
4. Sulfathiazole
5. Acebutolol
6. Dextromethorphan
7. Diphenhydramine
8. Propafenone
9. Amitriptyline
10. Fluoxetine
11. Naproxen
12. Diflunisal
13. Indomethacin
GL Sciences Inertsil
®
5 µm ODS-3 250 x 4.6 mm
Kinetex 2.6 µm C18 150 x 4.6 mm
App ID 18586
18586
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Increase in Sensitivity
Phenomenex
l
WEB: www.phenomenex.com