20
>
0
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140
160
mAU
20767
0
10
20 min
0
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160
mAU
20767
0
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20 min
Conditons for both columns
Column: Kinetex 5 µm C18
ZORBAX 5 µm XDB-C18
Dimensions: 250 x 4.6 mm
Mobile Phase: A: TFA/Water/THF (5:900:100)
B: TFA/Acetonitrile/THF (5:900:100)
Gradient: Time (min) % B
0
10
40
80
Flow Rate: 1.5 mL/min
Temperature: 40 °C
Detection: UV @ 295 nm
Sample: 1. Impurity A
2. Impurity B
3. Impurity C
4. Unidentified
5. Paroxetine
6. Impurity D
Agilent
®
ZORBAX
®
5 µm XDB-C18
Kinetex
®
5 µm XB-C18
App ID 20767
Agilent and ZORBAX are registered trademarks of Agilent Technologies, Inc. (Palo Alto, CA, USA). Phenomenex is not affiliated with Agilent Technologies. Comparative separa-
tions may not be representative of all applications.
0
20
40
60
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140
160
mAU
20766
0
10
20 min
a
b c
d
0
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40
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160
mAU
20766
0
10
20 min
b
b
c
c
Height = 120 mAU
Height = 165 mAU
*
*
R
S
= 0.98
R
S
= 2.23
*
*
Increased Resolution
and Sensitivity
By simply dropping in a
Kinetex 5 µm
for this paroxetine stability-indicating assay you get
an impressive enhancement in resolution and sensitivity. Now imagine what this column can
do for your other 3 and 5 µm methods.
TM
5
µm
App ID 20766
Phenomenex
l
WEB: www.phenomenex.com